Coding
Part:BBa_K3799000:Design
Designed by: Shubhamay Das Group: iGEM21_IISER_Kolkata (2021-09-30)
EiCsm6,A CRISPR system endoribonuclease
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 314
Illegal XhoI site found at 367
Illegal XhoI site found at 1054 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The gene is codon-optimized to have a good expression in E. Coli and is also easy to commercially synthesize with the IDT online codon optimizer.
Source
Enterococcus italicus, sequence found from https://www.uniprot.org/uniprot/E6LHV2