Coding

Part:BBa_K3799000:Design

Designed by: Shubhamay Das   Group: iGEM21_IISER_Kolkata   (2021-09-30)


EiCsm6,A CRISPR system endoribonuclease


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 314
    Illegal XhoI site found at 367
    Illegal XhoI site found at 1054
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The gene is codon-optimized to have a good expression in E. Coli and is also easy to commercially synthesize with the IDT online codon optimizer.


Source

Enterococcus italicus, sequence found from https://www.uniprot.org/uniprot/E6LHV2

References

https://www.addgene.org/115268/